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liter yeast extract  (Thermo Fisher)


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    Thermo Fisher liter yeast extract
    Liter Yeast Extract, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/liter+yeast+extract/Yeast+Extract/pmc12755901-266-27-40
    Average 96 stars, based on 1 article reviews
    liter yeast extract - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Drosophila Abi maintains blood cell homeostasis by promoting clathrin-mediated endocytosis of Notch
    Article Snippet: These cells were maintained at 25°C in Shields and Sang M3 Insect Medium (USBiological Life Sciences) supplemented with 10% heat-inactivated (30 min, 56°C) fetal bovine serum, 1 g/liter yeast extract, 2.5 g/liter Bacto-peptone, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco).

    Article Title: Selection of Autochthonous Saccharomyces cerevisiae Strains as Wine Starters Using a Polyphasic Approach and Ochratoxin a Removal
    Article Snippet: A semisynthetic medium with the following composition was used: 100 g liter glucose (C. Erba, Milan, Italy), 100 g liter fructose (Sigma-Aldrich, Milan, Italy), 10 g liter yeast extract (Oxoid), 1 g liter ammonium sulfate (J. T. Baker, Milan, Italy), 1 g liter potassium phosphate (C. Erba), and 1 g liter magnesium sulfate (J. T. Baker); a second test was run using 250 g/liter of reduced sugars by the addition of glucose:fructose (1:1).

    Article Title: Properties of whey protein-based films containing organic acids and nisin to control Listeria monocytogenes.
    Article Snippet: The cultures were maintained at 280uC in BBL Trypticase soy broth (Becton Dickinson, Sparks, MD) containing 10% (vol/vol) glycerol and subcultured twice at 37uC 18 to 24 h before use in tryptone soy broth with yeast extract and glucose (TSBYEG), which contained 30 g/liter tryptone soya broth, 3 g/liter yeast extract, and 10 g/liter dextrose (Oxoid, Basingstoke, UK).

    Article Title: Study on the use of bovine blood protein hydrolysate as a peptone in microbial culture media.
    Article Snippet: Livestock blood is a protein-rich waste byproduct produced during meat production processes in slaughterhouses.. Its utilization through conversion into value-added products is an intriguing management strategy.. In this study, bovine blood was used to obtain the protein hydrolysate for use as a peptone for microbial growth medium.



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    A: Schematic of a mating scheme for transfer of the Tn 7 transposon and Tn 7 transposase-expressing plasmid into the recipient of interest. B: Schematic of a mating scheme for delivery of a Tn 7 transposon into R. sphaeroides constitutively expressed with plasmid-encoded Tn 7 transposase genes ( tnsABCD ). C&D: Transposition efficiency into N. aromaticivorans and R. sphaeroides, respectively, using different mating schemes. E: Growth of N. aromaticivorans strains with or without att::Tn 7 insertions in rich <t>(464a)</t> and minimal (SIS+glucose) media. F: Growth of R. sphaeroides strains with or without att::Tn 7 insertions in rich (LB) and minimal (SIS) media. G: Stability of a kanR Tn 7 insertion in both N. aromaticivorans and R. sphaeroides following serial passaging in the absence of selection. N.a.=N. aromaticivorans , R.sp. = R. sphaeroides . Summary statistics and growth curve data can be found in tables S2-S5.
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    A: Schematic of a mating scheme for transfer of the Tn 7 transposon and Tn 7 transposase-expressing plasmid into the recipient of interest. B: Schematic of a mating scheme for delivery of a Tn 7 transposon into R. sphaeroides constitutively expressed with plasmid-encoded Tn 7 transposase genes ( tnsABCD ). C&D: Transposition efficiency into N. aromaticivorans and R. sphaeroides, respectively, using different mating schemes. E: Growth of N. aromaticivorans strains with or without att::Tn 7 insertions in rich (464a) and minimal (SIS+glucose) media. F: Growth of R. sphaeroides strains with or without att::Tn 7 insertions in rich (LB) and minimal (SIS) media. G: Stability of a kanR Tn 7 insertion in both N. aromaticivorans and R. sphaeroides following serial passaging in the absence of selection. N.a.=N. aromaticivorans , R.sp. = R. sphaeroides . Summary statistics and growth curve data can be found in tables S2-S5.

    Journal: bioRxiv

    Article Title: Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides

    doi: 10.1101/2023.08.25.554875

    Figure Lengend Snippet: A: Schematic of a mating scheme for transfer of the Tn 7 transposon and Tn 7 transposase-expressing plasmid into the recipient of interest. B: Schematic of a mating scheme for delivery of a Tn 7 transposon into R. sphaeroides constitutively expressed with plasmid-encoded Tn 7 transposase genes ( tnsABCD ). C&D: Transposition efficiency into N. aromaticivorans and R. sphaeroides, respectively, using different mating schemes. E: Growth of N. aromaticivorans strains with or without att::Tn 7 insertions in rich (464a) and minimal (SIS+glucose) media. F: Growth of R. sphaeroides strains with or without att::Tn 7 insertions in rich (LB) and minimal (SIS) media. G: Stability of a kanR Tn 7 insertion in both N. aromaticivorans and R. sphaeroides following serial passaging in the absence of selection. N.a.=N. aromaticivorans , R.sp. = R. sphaeroides . Summary statistics and growth curve data can be found in tables S2-S5.

    Article Snippet: N. aromaticivorans strains were grown in 464a (5g tryptone, 5g yeast extract, 1g glucose per liter) (DSMZ) or SIS supplemented with 1% glucose.

    Techniques: Expressing, Plasmid Preparation, Passaging, Selection

    A: 10-fold serial dilutions of N. aromaticivorans with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. N. aromaticivorans cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (464a) in the presence or absence of 1mM IPTG. B: 10-fold serial dilutions of R. sphaeroides with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. R. sphaeroides cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (LB) in the presence or absence of 1mM IPTG. Figure S3 shows additional biological replicates of each.

    Journal: bioRxiv

    Article Title: Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides

    doi: 10.1101/2023.08.25.554875

    Figure Lengend Snippet: A: 10-fold serial dilutions of N. aromaticivorans with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. N. aromaticivorans cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (464a) in the presence or absence of 1mM IPTG. B: 10-fold serial dilutions of R. sphaeroides with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. R. sphaeroides cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (LB) in the presence or absence of 1mM IPTG. Figure S3 shows additional biological replicates of each.

    Article Snippet: N. aromaticivorans strains were grown in 464a (5g tryptone, 5g yeast extract, 1g glucose per liter) (DSMZ) or SIS supplemented with 1% glucose.

    Techniques: Construct, Serial Dilution

    A: 10-fold serial dilutions of N. aromaticivorans with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. N. aromaticivorans cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (464a) in the presence or absence of 1mM IPTG. B: 10-fold serial dilutions of R. sphaeroides with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. R. sphaeroides cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (LB) in the presence or absence of 1mM IPTG. Samples presented in the main text are indicated in gray.

    Journal: bioRxiv

    Article Title: Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides

    doi: 10.1101/2023.08.25.554875

    Figure Lengend Snippet: A: 10-fold serial dilutions of N. aromaticivorans with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. N. aromaticivorans cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (464a) in the presence or absence of 1mM IPTG. B: 10-fold serial dilutions of R. sphaeroides with MCi constructs with a sgRNA targeting the essential gene murC or a non-targeting control sgRNA. R. sphaeroides cells were normalized to an OD600 of 10 prior to serial dilution. Cells were grown on rich media (LB) in the presence or absence of 1mM IPTG. Samples presented in the main text are indicated in gray.

    Article Snippet: N. aromaticivorans strains were grown in 464a (5g tryptone, 5g yeast extract, 1g glucose per liter) (DSMZ) or SIS supplemented with 1% glucose.

    Techniques: Construct, Serial Dilution

    A: Expression of crtW from the att::Tn 7 site in Δ crtG N. aromaticivorans produces comparable quantities of astaxanthin as a previously-engineered expression strain (Δ crtG :: crtW + ) . There is no difference in the amount of astaxanthin produced among all crtW strains; astaxanthin was not detectable in cells lacking this foreign gene (Δ crtG , Tn 7 construct “None”). B: 10-fold serial dilutions of Δ crtG :: crtW + cells with an MCi system at the att::Tn 7 site. Cells were plated on rich media (464a) lacking (B) or supplemented with (C) 1mM IPTG. n.d. = Not detected. Summary statistics can be found in table S20.

    Journal: bioRxiv

    Article Title: Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides

    doi: 10.1101/2023.08.25.554875

    Figure Lengend Snippet: A: Expression of crtW from the att::Tn 7 site in Δ crtG N. aromaticivorans produces comparable quantities of astaxanthin as a previously-engineered expression strain (Δ crtG :: crtW + ) . There is no difference in the amount of astaxanthin produced among all crtW strains; astaxanthin was not detectable in cells lacking this foreign gene (Δ crtG , Tn 7 construct “None”). B: 10-fold serial dilutions of Δ crtG :: crtW + cells with an MCi system at the att::Tn 7 site. Cells were plated on rich media (464a) lacking (B) or supplemented with (C) 1mM IPTG. n.d. = Not detected. Summary statistics can be found in table S20.

    Article Snippet: N. aromaticivorans strains were grown in 464a (5g tryptone, 5g yeast extract, 1g glucose per liter) (DSMZ) or SIS supplemented with 1% glucose.

    Techniques: Expressing, Produced, Construct